Calcein am propidium iodide protocol
Calcein am molecular weight Calcein and Propidium Iodide Assay Protocol: The calcein assay is based on the conversion of the cell permeant non-fluorescnt calcein AM dye to the fluorescent calcein dye by intracellular esterase activity in live cells. Propidium iodide (PI) is membrane impermeant and therefore does not enter viable cells with intact membranes.
Ethidium homodimer Protocol aim The aim of this protocol is to provide instructions for performing live dead staining using Calcein AM and Propidium iodide (PI) for imaging of live respectively dead cells in 3D constructs of CELLINK bioink, GelMA and GelX variations. Material needed - Stock solution of 1 mM Calcein AM* - Stock solution of 2 mg/ml PI** - Scale.
Ethidium homodimer-1
The aim of this protocol is to provide instructions for performing live and dead staining using Calcein AM and Propidium Iodide (PI) for imaging of live respectively dead cells in 3D constructs of all CELLINKs bioinks. Materials needed Calcein AM (Invitrogen™ eBioscience™, product number ). 
Calcein am staining Preparation of Calcein AM Calcein AM optimally excites at nm and has a peak emission at nm (use FL-1 channel). Some flow cytometers may require use of a FL-1 99% attenuation filter. Calcein AM is supplied as a lyophilized powder that may be slightly visible as an iridescent sheen inside the vial.
Research Centers: Johns Hopkins Medicine Description: This protocol describes the analysis of cell viability on A cells using Calcein AM as a measure of enzymatic activity in live cells, Propidium Iodide as a measure of dead cells with compromised membrane integrity and Hoechst as a marker for all nucleated cells.
Viability Dye Staining - Thermo Fisher Scientific In this section, we address the imaging protocol to quantitatively assess live and dead cell proportions in cancer cells after anticancer drug treatment (see Note 3). Materials 1. Calcein AM ( μM), Molecular Probes/Invitrogen #C 2. Propidium iodide (1 mg/mL in water), Molecular Probes #P (see Note 4). 3.
LIVE/DEAD Cell Viability Assays: Thermo Fisher Scientific - US Prepare a Calcein AM Staining Solution by adding 2 µl of Cell-Based Assay Calcein AM (Item No. ) to 10 ml of the culture medium used in your experiment. Mix well and protect from light. The Calcein AM Staining Solution will be stable for one hour at room temperature. NOTE: Protect from light. Propidium Iodide Solution.
Experimental Protocol for Calcein AM Assay - AAT Bioquest 5-carboxyfluorescein diacetate, acetoxymethyl ester (CFDA AM) Propidium iodide; Dimethylsulfoxide (DMSO), anhydrous; Detailed protocols (Product Information Sheet) ~ flow cytometry assays: The cell-permeant CFDA AM is combined with the membrane integrity indicator propidium iodide to evaluate the vitality of yeast cells by flow cytometry or.