Pfu polymerase temperature
PCR Fidelity of Pfu DNA Polymerase and Other Thermostable DNA The optimal extension temperature for Pfu DNA Polymerase is °C. The recommended extension step is 2 min/kb at 72°C for PCR products up to 2 kb. For larger products, the extension time should be prolonged by 1 min/kb. Number of cycles.
What is the Difference Between Taq Polymerase and Pfu Pfu. DNA Polymerase Protocol. Instructions for Use of Product (s) M, M Literature # 9PIM Pfu DNA Polymerase is a thermostable enzyme that replicates DNA at 75°C. It catalyzes the polymerization of nucleotides into duplex DNA in the 5´→3´ direction in the presence of magnesium.
Manual: Pfu DNA Polymerase -
However, Pfu is slower and typically requires 1–2 minutes per cycle to amplify 1kb of DNA at 72 °C. Using Pfu DNA polymerase in PCR reactions also results in blunt-ended PCR products. Pfu DNA polymerase is hence superior to Taq DNA polymerase for techniques that require high-fidelity DNA synthesis, but can also be used in conjunction with. 
Bacterial thermophilic DNA polymerases: A focus on prominent Average error rates of thermostable DNA polymerases were found to increase in the following order: Pfu ( × 10 −6).
Pfu DNA Polymerase Protocol - Promega Corporation melting temperature (Tm) of the primers. Annealing for 30 mutagenic base uracil by family B DNA polymerases seconds is normally sufficient. If non-specific PCR products appear, the annealing temperature should be optimized stepwise in °C increments. Extension The optimal extension temperature for Pfu DNA Polymerase is °C.
PfuTurbo DNA Polymerase - Agilent PfuUltrahigh-fidelity DNA polymerase exhibits optimal polymerase activity at 75°C and only 2–8% activity between 40–50°C. Taq DNA polymerase, however, exhibits optimal polymerase activity between 60–70°C and 27–70% activity between 40–50°C.
Construction, Expression, and Characterization of Recombinant Thermostable DNA polymerase is important in modern biotechnology and molecular biology because it results in methods such as DNA cloning, DNA sequencing, whole genome amplification, molecular diagnostics, polymerase chain reaction, synthetic biology, and single nucleotide polymorphism detection.
Pfu DNA Polymerase - VWR u. Pfu DNA Polymerase is a high-fidelity, thermostable enzyme of approximately 90kDa isolated from Pyrococcus furiosus. The enzyme replicates DNA at 75°C, catalyzing the polymerization of nucleotides in the 5´→3´ direction in the presence of magnesium. Pfu DNA Polymerase also possesses 3´→5´ exonuclease (proofreading) activity.